Instrument , Method , and Sample Optimizations to Get the Most from Agilent
نویسنده
چکیده
Six compounds were analyzed through isocratic elution to demonstrate the effect of several instrument, method, and sample variables on column performance. A very efficient Agilent InfinityLab Poroshell 120 EC-C18, 2.1 × 50 mm, 1.9 μm column capable of generating nearly 14,000 plates was used in this work. The impact on column performance of system capillaries, detector flow cells, data collection rates, injection volumes, sample solvents, and sample concentrations was studied.
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